Consensus Report Fetal Bovine Serum (FBS): Past – Present – Future

نویسندگان

  • Jan van der Valk
  • Karen Bieback
  • Christiane Buta
  • Brett Cochrane
  • Wilhelm G. Dirks
  • Jianan Fu
  • James J. Hickman
  • Christiane Hohensee
  • Roman Kolar
  • Manfred Liebsch
  • Francesca Pistollato
  • Markus Schulz
  • Daniel Thieme
  • Tilo Weber
  • Joachim Wiest
  • Stefan Winkler
  • Gerhard Gstraunthaler
چکیده

Jan van der Valk, Karen Bieback, Christiane Buta, Brett Cochrane Wilhelm G. Dirks, Jianan Fu, James J. Hickman, Christiane Hohensee, Roman Kolar, Manfred Liebsch, Francesca Pistollato, Markus Schulz, Daniel Thieme, Tilo Weber, Joachim Wiest, Stefan Winkler and Gerhard Gstraunthaler The 3Rs-Centre Utrecht Life Sciences, Utrecht University, Utrecht, The Netherlands; Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany; SET Foundation, Frankfurt a. M., Germany; Animal Free Research UK, Hitchin, Hertfordshire, England; Leibniz-Institute DSMZ, German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany; PAN-Biotech Ltd, Aidenbach, Germany; NanoScience Technology Center, University of Central Florida, Orlando, USA; Invitro+Jobs, The Federal Association of People for Animal Rights Germany (PARG), Aachen, Germany; Animal Welfare Academy, German Animal Welfare Federation, Neubiberg, Germany; Königs Wusterhausen, Germany; Directorate F – Health, Consumers and Reference Materials, Joint Research Centre, Ispra, VA, Italy; BASF SE, Experimental Toxicology and Ecology, Ludwigshafen, Germany; Department of Ophthalmology, Universität Erlangen-Nürnberg, Erlangen, Germany; cellasys GmbH, Kronburg, Germany; Institute of Applied Cell Culture (IAZ), Munich, Germany; Division of Physiology, Medical University Innsbruck, Innsbruck, Austria

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Fetal Bovine Serum (FBS): Past - Present - Future.

The supplementation of culture medium with fetal bovine serum (FBS, also referred to as "fetal calf serum") is still common practice in cell culture applications. Due to a number of disadvantages in terms of quality and reproducibility of in vitro data, animal welfare concerns, and in light of recent cases of fraudulent marketing, the search for alternatives and the development of serum-free me...

متن کامل

Enhancement in colonization of bovine spermatogonial stem cells following addition of knock-out serum replacement to culture medium

Enrichment of cell suspension with germ cells prior to injection into recipient seminiferous tubules is of importance in spermatogonial stem cells (SSCs) transplantation. Knock-out serum replacement (KSR) has been reported to enhance the proliferation of murine SSCs and human embryonic stem cells. The aim of the present study was to investigate the effect of KSR versus fetal bovine serum (FBS) ...

متن کامل

P-76: Stepwise Reduction of Fetal Bovine Serum Levels in Chinese Hamster OvaryCells -Expressing Human Chorionic Gonadotrophin- Culture

Background: The demand for producing recombinant therapeutic proteins by mammalian cell lines, such as Chinese hamster ovary (CHO) cells, continues to grow. Significant achievements in process optimization including development of cell culture strategies for largescale and cost-effective production have been made. Fetal bovine serum (FBS) is an often essential growth supplement and yet most cos...

متن کامل

Research Article: In vitro investigation on response of cultivated hepatocytes from Klunzinger\'s mullet, Liza klunzingeri, to temperature and fetal bovine serum

The appropriate temperature and growth supplementary factors such as fetal bovine serum (FBS) are the necessities needed for cell growth in culture medium. In this regards, the effect of Leibovitz-15 (L-15) medium containing different concentrations of fetal bovine serum (FBS) was assessed on growth and proliferation of cultivated liver cells of klunzingerchr('39')s mullet, Liza klunzingeri, in...

متن کامل

Effect of fetal and adult bovine serum on pyocyanin production in Pseudomonas aeruginosa isolated from clinical and soil samples

Objective(s): Pyocyanin is a blue-greenish redox-active pigment, produced by Pseudomonas aeruginosa, with a wide range of biological and biotechnological applications. Pyocyanin biosynthesis is regulated by the quorum-sensing (QS) system in which the expression of QS genes and QS-controlled virulence genes may be affected by serum as a complex medium. In the current study, effects of adult bovi...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2017